ChIP-seq of EsrB - SRA

SRX23813854: ChIP-seq of EsrB
1 ILLUMINA (Illumina HiSeq 1500) run: 7.5M spots, 1.1G bases, 348.8Mb downloads

Design: Cross-linking was performed with formaldehyde, followed by quenching with glycine. Bacterial pellets were collected, washed with pre-cooled IP buffer, and resuspended for high-pressure disruption. Sonication was carried out, and the samples were then de-crosslinked. Chromatin immunoprecipitation was conducted using specific antibodies, followed by stringent washing steps. High-throughput sequencing libraries were prepared from 10 ng DNA.

Submitted by: East China University of Science and Technology

Study: ChIP-seq of EsrB

PRJNA1082872 • SRP492924 • All experiments • All runs

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To explore the regulatory role of EsrB in Edwardsiella tarda across different environmental contexts, we conducted ChIP-seq analyses to elucidate EsrB's DNA binding patterns in LB and DMEM environments.

Sample: WT:ESRB(LB)

SAMN40219304 • SRS20636449 • All experiments • All runs

Organism: Edwardsiella piscicida

Library:

Name: 8

Instrument: Illumina HiSeq 1500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Runs: 1 run, 7.5M spots, 1.1G bases, 348.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR28200766	7,547,324	1.1G	348.8Mb	2024-11-04

ID:: 32115369

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